Understand the basis of activity dependant labeling in systems Neuroscience

Author: NMJ

Created: 2021-09-28 03:22am

Edited: 2021-09-28 08:10pm

Keywords: immediate early genes, transgenic animal models, activity dependant promoters

Description:

In this activity, we will explore and learn the different options we have to look at the activity of brain cells upon specific behaviors.

Intended Learning Outcomes:

Study the cellular and molecular basis of the three types of activity dependant labelings available
Develop a protocol to label active cells after fear conditioning
Analyse the results of the staining and quantification of the active cells
Write a report following the academic and scientific codes

	Resources		Tasks		Supports
	Articles, book chapter	→	Learn about the activity dependant systems available for systems neuroscience ↓	←	Face-to-face interaction, Journal Club
←	Protocol	←	Write a protocol we could use to label cells activated by a fear conditioning protocol ↓	←	Face-to-face interaction, support during lab work
←	The student plots graphs from the analysis and write a result section	←	Collect and analyse data ↓	←	Face-to-face interaction, on-line support
←	Report written by the student	←	Write a report including the experimental methodology, the results, a discussion, and a summary of the different applications possible to answer our specific questions	←	Face-to-face interaction, on-line support, follow-up on their report

I will use a padlet to ease the communication and material exchange with the student.