Microbial growth - analysis of lab data
Description:
This exercise deals with the augmentation of a Bachelor course on microbiology, in the specific on a module on microbial growth where students have to analyze and interpret results obtained from laboratory exercises performed on the previous week. Regularly issues emerge with students (at their first year) not being proficient with Excel. This requires frequent interruption of the data analysis process to explain some basic statistic and analytic tools. Such explanation breaks generate nuisance among the most proficient students, interrupt the flow of the data analysis, and leave limited time for the interpretation and discussion of the results.
Here following the principle of the STREAM model, I create an out-of-class loop, where students can access contents (i.e., a document and a video) on statistical concepts and Excel analytical commands. Then, groups of three-four students will conduct a calculation exercise on a provided Excel template, individually. Finally, students will report the results of their calculation by answering to a quiz. Students will get immediate feedback on the correctness of the answer. If one answer is incorrect, students will swap the calculation spreadsheet and conduct a “peer review”. They can then take the quiz again. Following the principle of Just in time teaching, I will look at the results before the lecture and consider if it will be necessary to go through those concepts again.
Intended Learning Outcomes:
- Learn basic principles and commands to carry out a regression analysis on Excel
- Read and interpret summary statistics
Resources | Tasks | Supports | |||
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Out of class |
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Document available on Brightspace |
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Read about principles of regression analysis ↓ |
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Teacher uploads the text |
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Video on Brightspace |
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Watch video on performing regressions on Excel ↓ |
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Teacher uploads Video |
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Excel file on Brightspace |
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Perform regression on Excel ↓ |
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Teacher uploads Excel with 'dummy' dataset |
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Questionnaire available on Brighspace |
Report results by answering a questionnaire ↓ |
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Teacher provide the questionnaire |
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In class |
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Excel spreadsheet pre-compiled with raw experimental data from lab exercise |
Calculate: 1) growth curves based on culture-dependent and culture-independent methods; 2) overall culture microbial activity; and 3) cell-specific microbial activity. ↓ |
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Data analysis performed under guidance of the teacher using screen-sharing. |
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Lecture on Microbial growth prior to the lab |
Identify phase of microbial growth and discuss pro and cons of culture-dependent and culture-independent methods |
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Data interpreted under guidance of the teacher |