Microbial growth - analysis of lab data

Author: Ugo

Created: 2022-09-26 10:33pm

Edited: 2022-09-28 08:35pm

Keywords: Microbial growth, activity, batch culture, cell density

Derived from: Microbiology - Growth in batch culture

Description:

This exercise deals with the augmentation of a Bachelor course on microbiology, in the specific on a module on microbial growth where students have to analyze and interpret results obtained from laboratory exercises performed on the previous week. Regularly issues emerge with students (at their first year) not being proficient with Excel. This requires frequent interruption of the data analysis process to explain some basic statistic and analytic tools. Such explanation breaks generate nuisance among the most proficient students, interrupt the flow of the data analysis, and leave limited time for the interpretation and discussion of the results.

Here following the principle of the STREAM model, I create an out-of-class loop, where students can access contents (i.e., a document and a video) on statistical concepts and Excel analytical commands. Then, groups of three-four students will conduct a calculation exercise on a provided Excel template, individually. Finally, students will report the results of their calculation by answering to a quiz. Students will get immediate feedback on the correctness of the answer. If one answer is incorrect, students will swap the calculation spreadsheet and conduct a “peer review”. They can then take the quiz again. Following the principle of Just in time teaching, I will look at the results before the lecture and consider if it will be necessary to go through those concepts again.

Intended Learning Outcomes:

  • Learn basic principles and commands to carry out a regression analysis on Excel
  • Read and interpret summary statistics
Resources Tasks Supports

Out of class

Document available on Brightspace

Read about principles of regression analysis

Teacher uploads the text

Video on Brightspace

Watch video on performing regressions on Excel

Teacher uploads Video

Excel file on Brightspace

Perform regression on Excel

Teacher uploads Excel with 'dummy' dataset

Questionnaire available on Brighspace

Report results by answering a questionnaire

Teacher provide the questionnaire

In class

Excel spreadsheet pre-compiled with raw experimental data from lab exercise

Calculate: 1) growth curves based on culture-dependent and culture-independent methods; 2) overall culture microbial activity; and 3) cell-specific microbial activity.

Data analysis performed under guidance of the teacher using screen-sharing.

Lecture on Microbial growth prior to the lab

Identify phase of microbial growth and discuss pro and cons of culture-dependent and culture-independent methods

Data interpreted under guidance of the teacher